Acta Botanica Boreali-Occidentalia Sinica
1000-4025
2024
44
2
288
299
10.7606/j.issn.1000-4025.20230524
article
基于表型性状构建材用云南松核心种质策略研究
Study on the Construction Strategy of Core Germplasm of Pinus Yunnanensis for Timber-used Based on Phenotypic Traits
【目的】研究旨在构建可靠的材用云南松核心种质，加强其种质资源选育、开发利用研究，解决其种质资源分布广、保存成本高、保存难度大等问题，促进云南松种质资源有效利用。【方法】以云南松26个天然居群的780株样株为原种质，以18个表型性状为原数据，利用2种不同构建策略（利用地理角度与改进的最小距离逐步取样法），探讨不同构建策略所构建的核心种质对原种质的代表性。【结果】结果表明：（1）地理角度构建出的包含219株样株的种质子集，其遗传多样性指数显著低于改进的最小距离逐步取样法构建的4个种质子集，略高于原种质；该种质子集与原种质的MD值为3.921%，VD值为83.33%，CR值为82.207%，VR值为99.48%；通过对原种质与该种质子集18个性状做主成分分析，累计贡献率分别为79.376%、82.163%，其种质子集样株分布相对集中。（2）改进的最小距离逐步取样法构建的4个抽样比例分别为10%、20%、30%和40%的种质子集中，其中20%抽样比例的种质子集效果最好，该种质子集的多样性指数极显著大于原种质；20%抽样比例种质子集与原种质的MD值为6.363%，VD值为83.33%，CR值为91.099%，VR值为124.448%；对20%抽样比例的种质子集进行主成分分析，累计贡献率为83.539%，且高于原种质，该种质子集样株分布范围覆盖了整个取样范围。【结论】研究表明，不同方法构建的核心种质均获得了原种质不同程度遗传多样性，这2种构建结果均可代表材用云南松种质资源核心种质；而地理角度构建的种质子集在种质资源采集、保存和更新方面更具有优势，可为材用云南松种质资源保存和优良种质选育提供科学方法，同时为其他种质资源的构建提供一种新的参考方法。
【Objective】 This study aims to construct a reliable core germplasm of Pinus yunnanensis for timber-used, enhance the research on its breeding, development, and utilization, and solve the challenges of its wide distribution, high preservation cost, and preservation difficulties, thereby promoting its effective utilization. 【Methods】 The original germplasm was derived from 780 sample plants of 26 natural P. yunnanensis populations, with 18 phenotypic traits serving as the original data, the study used 18 phenotypic traits as the original data and employed two distinct construction strategies (geographical perspective and an enhanced minimum distance stepwise sampling method) to assess the representativeness of the core germplasm constructed using different strategies. 【Results】 The findings indicated that: (1)The genetic diversity index of the germplasm subset, which included 219 samples constructed from a geographical perspective, was significantly lower than that of the four subsets constructed by the enhanced minimum distance stepwise sampling method, however, it was slightly higher than the original germplasm. The germplasm subset and the original germplasm had MD value of 3.921%, VD value of 83.33%, CR value of 82.207%, and VR value of 99.48%. A principal component analysis of the original germplasm quality and the germplasm subset''s 18 traits revealed cumulative contribution rates of 79.376% and 82.163%, respectively, the germplasm subset distribution was relatively concentrated. (2)The enhanced minimum distance stepwise sampling method was utilized to construct four seed proton sets with sampling ratios of 10%, 20%, 30%, and 40%. Among these, the germplasm subset with a 20% sampling ratio proved to be the most effective, with a diversity index significantly higher than the original germplasm. The germplasm subset with 20 % sampling proportion and the original germplasm had MD value of 6.363%, VD value of 83.33%, CR value of 91.099%, and VR value of 124.448%. A principal component analysis was conducted on a germplasm subset with a 20% sampling proportion, revealing a cumulative contribution rate of 83.539%, which exceeded that of the original germplasm. The germplasm subset distribution range encompassed the entire sampling range. 【Conclusion】 The study demonstrates that core collections, constructed using different methods, yield varying degrees of genetic diversity from the original germplasm, while both construction methods could represent the core collection of P. yunnanensis germplasm resources. The germplasm subset derived from the geographical perspective offers more advantages in terms of collection, preservation, and renewal of these resources, this approach could provide a scientific method for the preservation of P. yunnanensis germplasm resources and the breeding of superior germplasm, offering a novel reference method for the construction of other germplasm resources.
云南松;核心种质;地理角度;Arcgis;表型性状
Pinus Yunnanensis ; core germplasm; geographical factors; Arcgis; phenotypic traits
李华,付朴艳,闫熙,曹子林,王晓丽
LI Hua,FU Puyan,YAN Xi,CAO Zilin and WANG Xiaoli
1.西南林业大学 林学院;2.西南林业大学 生态与环境学院
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