Based on the EST sequence encoded the glutathione S-transferase (GST),gene-specific primers were designed and used to obtain the full-length cDNA of a novel GST gene from Pyrus pyrifolia Nakai cv.Huobali in Yunnan Province with the method of rapid amplification of cDNA ends (RACE).This novel gene was named as PpGST.PpGST is 1 177 bp in length with an ORF of 696 bp,a 5′-untranslated region (UTR) of 130 bp,and a 3′-UTR of 351 bp,and the ORF encodes a predicted polypeptide of 231 amino acids.A phylogenetic analysis of the relationship of the newly identified PpGST with some known plant GSTs grouped the PpGST into the class of zeta GSTs.PpGST is abundantly expressed in pericarps of Huobali regardless received sunlight or not,but no expression was detected in the young leaves.The abundant expression of PpGST in fruits of P.pyrifolia hints that the PpGST may be involved in maintaining the redox balance and stress response during the pear fruit development.