The bud and leaf of Photinia fraseri were used to study the effect of hormone and cultivation condition on callus induction and plantlet regeneration.The results indicated that the best medium for callus induction was MS+0.10 mg/L 2,4-D+0.50 mg/L NAA+0.50 mg/L 6-BA+0.50 mg/L KT.The callus induction rate under dark culture kept higher than that of light culture.The callus induction rates were 100%（bud） and 98%（leaf）,respectively.The best medium for proliferation was MS+0.50 mg/L IBA+2.00 mg/L 6-BA+2.00 mg/L KT.The differentiation rate was more than 91% and multiplication times were more than 6.8.The best medium for roots formation was 1/2MS+0.50 mg/L IBA+0.01 mg/L NAA,and the rooting rate was 90.0%.The rooting number was 4.4 per plant.