限氮培养对小球藻水分含量和叶绿素荧光参数的影响
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农业部核农学重点开放实验室基金项目(KYJD09017);四川省科技厅应用基础研究项目(2009JY0144)


Effects of Nitrogen-limited Culture on Water Content and Chlorophyll Fluorescence Parameters in Chlorella sorokiniana
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    摘要:

    限氮培养是提高小球藻油脂含量的一种方法,本研究探讨了小球藻限氮培养过程中藻细胞的生物量、水分含量和叶绿素荧光参数的变化。结果表明:在限氮培养条件下,小球藻藻细胞的生物量和水分含量分别下降了18%和7%;藻细胞的最大光能转化效率(Fv/Fm),表观量子效率(α)和最大光合电子传递速率(rETRmax)在整个限氮培养过程中均快速下降,到培养末期下降至接近于0,显著低于对照;半饱和光强(Ek)在对数期迅速下降,到稳定期后显著上升;最小荧光(F0)在整个限氮培养过程中显著上升。可见,限氮培养显著影响了小球藻细胞光系统Ⅱ的结构和功能。

    Abstract:

    Nitrogen-limited culture is a kind of method improving the oil content of Chlorella sorokiniana.The biomass,water content and fluorescence parameters of chlorella cultivated under nitrogen-limited medium were investigated.The results showed that the biomass and water content of chlorella under nitrogen-limited culture declined 18% and 7%,respectively;the maximal photochemical efficiency of PSⅡ (Fv/Fm),apparent quantum efficiency (α) and maximum photosynthetic electron transport rate (rETRmax) rapidly fell during the course of nitrogen-limited culture,and fell to close zero by the later period of culture;the minimum saturating irradiance (Ek,light-resistant index) firstly rapidly decreased from the early logarithmic period,and then remarkably increased during stable period;the minimum fluorescence(F0) obviously increased during the nitrogen-limited culture.These results showed that nitrogen-limited culture significantly influenced the structure and function of PSⅡ in chlorella.

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陈元印,唐运来,陈 梅.限氮培养对小球藻水分含量和叶绿素荧光参数的影响[J].西北植物学报,2012,32(3):491-497

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  • 在线发布日期: 2012-10-31
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