GFP gene was amplified by PCR using the plasmid of pMCB30 as template,and the corrected GFP fragment was ligated into the plant expression vector of pCMBIA2300-35S-OCS.The p35S:GFP vector was transformed into Agrobacterium GV3101,then p35S:GFP transferred into Olimarabidopsis pumila and Arabidopsis thaliana through Agrobacterium-mediated method.2 independent plants of T₁ generation of transgenic O.pumila and 9 independent plants of T₁ generation of transgenic A.thaliana were screened on 1/2 MS medium containing kanamycin.GFP protein can be detected in the root tip cells of transgenic O.pumila and A.thaliana by confocal laser microscopy.GFP gene was confirmed by PCR amplification using DNA of transgenic plants,indicated that GFP gene had been successfully transformed into transgenic plants.The study layed foundations for further using of GFP gene and set up the genetic transformation system of O.pumila,which will benefit a lot in further study of the functional genes of O.pumila.