小麦盐胁迫相关基因的克隆与表达分析
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新疆维吾尔自治区高技术研究发展项目(201011109);新疆维吾尔自治区高校科研启动项目(XJEDU2011S20);新疆农业大学校前期资助课题(XJAU201019);作物遗传育种重点学科资助项目


Cloning and Expression Analysis of a Salt Stress Responsive Gene from Triticum aestivum
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    采用RTPCR方法,从小麦中克隆获得1个盐诱导小麦MYB类转录因子基因TaSIM(Triticum aestivum saltinduced MYB),该基因cDNA全长1 213 bp,具有1个831 bp的开放阅读框,编码276个氨基酸,预测分子量约为29.903 kD,等电点为10.12,推测的氨基酸序列中含有2个高度保守的SANT结构域。系统发生树分析表明,TaSIM与二穗短柄草XP_003576185亲缘关系最近。半定量RTPCR检测结果显示,TaSIM基因受盐胁迫诱导表达。亚细胞定位结果显示,TaSIMhGFP融合蛋白定位于细胞核中。研究结果表明,小麦TaSIM基因编码的蛋白可能在细胞核内参与小麦对盐胁迫的应答反应。

    Abstract:

    In this study,a saltinduced MYB transcription factor gene,TaSIM,was isolated from cDNAs library of wheat leaves under salt stress.The full length TaSIM cDNA is 1 213 bp,including an ORF of 831 bp.This cDNA sequence encoded a polypepide of 276 amino acid residues with a predicted molecular mass of 29.903 kD and a basic isoelectric point of 10.12,and two highly conserved SANT domain in the encoded putative protein.The phylogenetic analysis showed that TaSIM has the highest similarity with XP_003576185 from Brachypodium distachyon.Semiquantitative RTPCR analysis revealed that TaSIM gene was obviously induced by salt stress.Subcellular localization assay indicated the TaSIMhGFP fusion protein was located in the nucleus.The results suggested that TaSIM transcription factor might be involved in salt stress response of wheat.

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于月华,王莉萍,高文伟,等.小麦盐胁迫相关基因的克隆与表达分析[J].西北植物学报,2012,32(6):1073-1078

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  • 在线发布日期: 2012-07-06
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