拟南芥钙调素结合蛋白参与胁迫响应的研究
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

内蒙古农业大学科技创新团队培育计划(NDPYTD20103);教育部新世纪优秀人才支持计划(NCET080871);国家自然科学基金(30860030)


Study on Arabidopsis thaliana Calmodulinbinding Protein Responding to Multiple Stresses
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    采用实时荧光定量RTPCR和Northern blotting技术检测了野生型拟南芥中CBP60g基因对丁香假单胞菌和非生物胁迫的响应,并对丁香假单胞菌接种后,野生型拟南芥、cbp60g1突变体和CBP60g过表达转基因植物中抗逆相关基因的表达变化进行检测。结果显示:(1)在野生型拟南芥中CBP60g基因的表达能被丁香假单胞菌、高盐、冷和机械损伤所诱导。(2)经丁香假单胞菌诱导后病程相关基因PR5和AIG1的表达在过表达转基因植物中明显高于野生型。(3)受干旱和ABA诱导的AtMYB2基因的表达在过表达转基因植物中也高于野生型。研究表明,CBP60g同时参与了拟南芥对生物和非生物胁迫响应。

    Abstract:

    In this study,realtime PCR or Northern blotting was employed to analyze the expression patterns of the CBP60g gene in wildtype Arabidopsis thaliana after Pseudomonas syringae and abiotic stress treatment.In addition,the expression of pathogeninduced genes in wildtype,cbp60g1 mutant and CBP60g overexpression plants after P.syringae inoculation were compared.The results showed:(1)Transcript of CBP60g was induced by P.syringae,high salt,cold and wounding treatment in wildtype plants.(2)The expression of pathogeninduced genes PR5 and AIG1 in the CBP60g overexpression plants was enhanced after P.syringae inoculation and was reduced in the mutant.(3)AtMYB2,an abioticstress induced gene reported previously,was also induced by Pst DC3000,and reached to a higher level in overexpression plants than in wildtype.Taken together,these results indicate that CBP60g involved in A.thaliana multiple stresses response.

    参考文献
    相似文献
    引证文献
引用本文

万东莉,丛靖宇,李瑞丽,等.拟南芥钙调素结合蛋白参与胁迫响应的研究[J].西北植物学报,2012,32(6):1099-1105

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2012-07-06
  • 出版日期:

微信公众号二维码

手机版网站二维码