The study was intended to discuss suitable spraying amount of Mg,Zn,Mo for high yield and good quality of leafuse ginkgo.Effects of spraying different levels of Mg,Zn,Mo fertilizers on leaf yield and medicinal quality of twoyearold ginkgo seedlings were tested by indoor stimulation experiment and pot experiment.The main results were submitted as fellows:（1）Combining application of Mg,Zn,Mo fertilizers increased leaf thickness,leaf area,single plant leaf area,leaf biomass.The best treatment was Mo3（2% Mg+0.3% Zn+0.1% Mo） increasing 64.9% leaf biomass compared with that of control.(2)Positive effect of combining Mg with Zn,Mo fertilizers on chlorophyll,soluble sugar and soluble protein contents decreased with the increasing of Mg concentration.The same was also the effects of combining Zn with Mg,Mo fertilizers and combining Mo with Mg,Zn fertilizers on chlorophyll,soluble sugar contents.In contrast,the effects on soluble protein content correlated positively with Zn or Mo concentration.(3)Combining application of Mg,Zn,Mo fertilizers increased total flavonoid and terpene lactones contents,total flavonoid and terpene lactones yields per plant.The best treatment was Mo3 increasing 296% total flavonoid yield per plant and 170% total terpene lactones yield per plant compared with that of control.(4)High concentration fertilizers as Mg4,Zn4 and Mo4 decreased positive effect combining application of Mg,Zn and Mo fertilizers on some indexes in different degree..The results indicate that appropriate combined fertilization management can increase chlorophyll,soluble sugar,soluble protein contents and improve ginkgo growth and medicinal quality which increase component factors of leaf yield and medicinal quality.In the experiment,Mo3 was the perfect combination in order to increase ginkgo leaf yield and medicinal composition content and yield.