Effects of culture medium composition on callus induction,shoot regeneration and root formation from cotyledon explants in two kinds of chicory(Puna chicory and Commander chicory) were evaluated to optimize the plant regeneration system.The Aeluropus littoralis Na⁺/H⁺ antiporter (AlNHX) gene,which encodes a Na⁺/H⁺ antiporter,was introduced into chicory to evaluate their genetic transformation efficiency.The results showed that the callus induction and shoot regeneration varied from chicory genotypes.The optimum medium for Puna chicory and Commander chicory were MS+1.5 mg/L 6-BA+0.2 mg/L IBA and MS+1.0 mg/L 6-BA+0.5 mg/L NAA,respectively.The optimum medium for Puna and Commander chicory root formation was 1/2MS+0.1 mg/L NAA.The insertion of AlNHX gene into chicory genome was confirmed by PCR.The genetic transformation efficiency of Puna chicory and Commander chicory were 10.0% and 13.3%,respectively.