西瓜防御素基因ClPDF2.1的克隆及表达分析
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家西甜瓜产业技术体系(CARS-NO.8);江苏省农业科技自主创新基金[cx(11)1001];江苏省农业科学院博士后基金(006046511105)


Cloning and Expression Analysis of Plant Defensin-like Gene (ClPDF2.1) from Watermelon
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    该研究从西瓜中克隆了西瓜防御素基因ClPDF2.1(GenBank登录号为KC481267)。序列分析结果表明,ClPDF2.1基因开放阅读框为225 bp,编码75个氨基酸,预测蛋白质分子质量为8.237 kD,等电点为9.375。蛋白质结构分析表明,ClPDF2.1蛋白含有防御素特有的8个半胱氨酸结构域。进化分析显示,ClPDF2.1与拟南芥PDF2归为一类,与五彩椒的亲缘关系最近(59%)。荧光定量PCR分析表明,ClPDF2.1在西瓜根、茎、叶器官中都有表达,叶中表达量高于根,茎中表达量最低。ClPDF2.1基因的表达也受到外源植物激素水杨酸、茉莉酸甲酯、乙烯利和西瓜枯萎病菌的诱导上调,表明ClPDF2.1基因通过信号传导途径参与对西瓜枯萎病菌株的防御反应。西瓜防御素基因的克隆及表达分析为其功能鉴定奠定了基础。

    Abstract:

    In this work,a defensin-like cDNA sequence was cloned from watermelon,named ClPDF2.1 (GenBank accession No.KC481267).Bioinformatics analysis showed that ClPDF2.1 encodes a putative polypeptide of 75 amino acids with a calculated molecular mass of 8.237 kD and a theoretical pI of 9.375.Sequence alignment showed that ClPDF2.1 had high homology to known PDF proteins from other plant species and contained the conserved eight cysteines motif.Phylogenetic analysis indicated that ClPDF2.1 belonged to the Arabidopsis PDF2 cluster,and was close to Capsicum annuum PDF gene with an overall sequence identity of 59%.Real-time PCR analysis revealed that ClPDF2.1 expressed in all tissues examined,with the highest in leaves,second in roots and the lowest in stems.Expression profiles under different treatments such as abscisic acid (SA),methyl jasmonate (JA),ethephon (ETH) and Fusarium wilt were compared,and the results revealed that transcriptional level of ClPDF2.1 was obviously up-regulated.These results suggested that ClPDF2.1 is probably employed the signal pathways to defense against Fusarium wilt pathogen.

    参考文献
    相似文献
    引证文献
引用本文

张 曼,羊杏平,王薇薇,等.西瓜防御素基因ClPDF2.1的克隆及表达分析[J].西北植物学报,2013,33(5):872-877

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2013-06-05
  • 出版日期:

微信公众号二维码

手机版网站二维码