秋水仙素诱导不结球白菜叶片形态类型与染色体加倍的关系
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江苏省科技支撑项目(BE2011313)


Relationship between Leaf Morphological Type and Chromosome Doubling of Non-heading Chinese Cabbage Induced by Colchicine
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    摘要:

    以二倍体不结球白菜‘依伶’为材料,用0.2%秋水仙素溶液处理子叶期生长点,研究不结球白菜在形态学上对秋水仙素诱导的响应及动态变化。结果表明:(1)‘依伶’对秋水仙素诱导的响应均以幼苗期前4片真叶中出现变异特征叶的形式表现,诱导处理15 d后出现了圆盘状、豆瓣状、棒状3种响应类型;处理30 d后,以上3种类型分别分化出长勺状、花状、圆锥状类型。(2)分化前后3种响应类型受秋水仙素抑制程度不同导致生长速率各不相同,生长速率的顺序分别为:圆盘状>豆瓣状>棒状,花状>长勺状>圆锥状,6种响应类型的特征叶逐渐萎蔫死亡,继续生长的其他.叶生长正常。(3)豆瓣状、圆盘状、长勺状、棒状、花状、圆锥状6种叶片形态响应类型于处理当代选株中四倍体加倍率依次分别为50.00%、41.67%、25.00%、16.67%、8.33%、0.00%。研究表明,早期筛选宜选用圆盘状、豆瓣状变异植株。

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    With diploid non-heading chinese cabbage ‘Yiling’ as material,0.2% colchicines was dropped in growth point of cotyledon stage.We studied non-heading Chinese cabbage response on colchicine induced and dynamic change in morphology.The results showed that:(1)‘Yiling’ response on colchicine induced is that variation of leaf appear in the first four leaves of seedling stage.After 15 days of treatment,we observed three response types:disc character,bean character and coliform character.After 30 days of treatment,disc character,bean character and coliform character is developed macro-scoop character,flower character and conical character respectively.(2)The above six response types have different degrees of inhibition of seedling stage:disc character>bean character>coliform character,flower character>macro-scoop character>conical character;the variation characteristic leaf wilting and died gradually,other variation leaves grow normally.(3) disc character,bean character,coliform character,macro-scoop character,flower character and conical character,Rate of tetraploid of six responsed types above is 50.00%,41.67%,25.00%,16.67%,8.33% and 0.00%,repectively,disc character and bean character should be used for early screening.

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徐丽娟,张蜀宁,郑于莉,等.秋水仙素诱导不结球白菜叶片形态类型与染色体加倍的关系[J].西北植物学报,2013,33(6):1239-1244

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  • 在线发布日期: 2013-07-07
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