黄精种子萌发过程发育解剖学研究
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中药材黄精规范化种植基地建设中的相关技术研究(陕西步长制药有限公司)


Developmental Anatomy of Polygonatum sibiricum Red.during the Seed Germination Process
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    摘要:

    采用石蜡切片技术对成熟黄精种子形态及萌发过程中的形态学变化及解剖结构特征进行了研究,以阐明黄精种子繁殖的生物学机制。结果显示:(1)成熟的黄精种子由外而内依次为种皮、胚乳和胚等3部分组成。其中种皮由一层木质化的细胞组成;胚乳占据种子的大部分结构,胚乳细胞含有大量淀粉,细胞壁增厚;胚处于棒型胚阶段。(2)黄精种子在萌发过程中棒型胚靠近种脐端分化为吸器、子叶联结和子叶鞘,靠近种孔的部位分化出胚根、胚轴和胚芽。(3)黄精种子萌发首先由子叶联结伸长将胚芽和胚根原基推出种孔,紧接着下胚轴膨大形成初生小根茎,吸器留在种子中分解吸收胚乳中的营养物质。(4)通过子叶联结连通吸器和初生小根茎,将胚乳中的营养物质由吸器-子叶联结这个通路转移到初生小根茎中,为初生根茎上胚芽和胚根的进一步分化提供物质保障。(5)黄精种子自然条件下萌发率较低,而且当年不出土。研究表明,黄精种子的繁殖生物学特性是其生态适应的一种重要机制。

    Abstract:

    To elucidate the biological mechanism of Polygonatum sibiricum seed propagation,we applied paraffin sections technology and safranine-fast green dyeing in studying mature seed morphology and morphological changes and the anatomy structure characteristics of germinating seeds.The result showed:(1)Mature P.sibiricum seed consists of seed coat,endosperm and embryo.The seed coat is a layer of lignified cells;the endosperm occupies most of the seed containing a large number of starch;the embryo stays in the club-shaped stage.(2)In germinating seed,sections near the embryo hilum would differentiate into haustorium,cotyledon coupling and cotyledonary node.Sections near the micropyle would differentiate into radicle,hypocotyls and germ.(3)At germination,cotyledon coupling stretch and push out the germ and radicle,then hypocotyl swells and forms newborn rhizome,haustorium degraded and absorbed nutrient substance of the seed.Cotyledon coupling are connected with haustorium and newborn rhizome,which transferred nutrient materials from endosperm to newborn rhizome.This pathway provides material assurance for the differentiation of the germ and radicle on the newborn rhizome.P.sibiricum seed germination rates is low under natural conditions,and it does not emergence until the next year.In summary breeding characteristics of P.sibiricum is an important mechanism adapting the environment.

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王剑龙,常 晖,周仔莉,等.黄精种子萌发过程发育解剖学研究[J].西北植物学报,2013,33(8):1584-1588

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  • 在线发布日期: 2013-08-27
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