In order to get high fresh-keeping botanical materials and develop new botanical preservativation,we determined the fresh-keeping activities of 35 Chinese herbal medicine bought in the market and optimal concentrations on the 28th day after storage in room temperature (RT) against tomatoes.Plants were extracted with ethanol by ultrasonic wave extraction.The fresh-keeping activity of the each plant extracts was assayed on Jinpeng tomato by spraying method.The control group (CK) was treated with clear water.The drug control was treated with 1-methylcyclopropene (1-MCP) fumigating 24 h airtightly.The results indicated that Litsea cubeba extract,pomelo peel extract and lotus leafs extract had higher fresh-keeping activities of them significantly during the storage,and the optimum concentrations were 2.0,2.0,1.0 mg·mL^-1 dry matter respectively.The treatments significantly reduced the weight-loss rate and the number of rotted tomatoes,slowed down the decrease in the flesh firmness effectively,titratable acidity,flavor,Vc,delayed the increase in total soluble solids,increased the superoxidae dismutase,catalase and peroxidase activities,restrained the increase of relative electric conductivity.The preservation effects were not significantly difference with antistaling agent 1-MCP and require further research.