In this paper,we isolated the diacylglycerol acyltransferase (DGAT) from Brassica napus and named BnDGAT1 gene.The amino acid sequence,protein domains and phylogenetic tree were analyzed.The BnDGAT1 protein was predicted that the amino sequences were composed of several completely functional domains,and eight hydrophobic transmembrane structures were detected by SOSUI.Phylogenetic tree analysis indicated that BnDGAT1 were closer with BjDGAT1,AtDGAT1 and TmDGAT1 than with other plant.In addition,expression profiles of BnDGAT1 were respectively analyzed using real-time PCR assays for different organization tissues and development stages of rape pod.Tissue-specific expression showed that the transcription expression levels in leaf and flower were higher than that in root and stem.Moreover,the expression levels of RNA transcription of pod were gradually down-regulated at different developmental stages of pod maturation.