The miR390-targeted TAS3 gene named FaTAS3 was isolated from strawberry (Fragaria ananassa ‘Allstar’) with the methods of PCR and RACE.Sequence analysis showed that the cDNA was consisted of a 742 base pair with a 16 bp poly A and highly conserved domains,two ta-siRNA-generating regions and one miR390 complementary site.The full-length DNA of FaTAS3 is 824 bp with an intron sequence of 98 bp at the 130 bp from the 5′ end of TAS3 transcript.In addition to the TATA/CAAT-box,its promoter predicted by bioinformatic analyses also contained some specific regulatory elements such as G-box,C-box and etc.The results of real time RT-PCR showed that the expression pattern of miR390 and its targeted TAS3 gene in strawberry is a similar manner to Arabidopsis.So we deduce that the biological formation of strawberry TAS3 gene was also cleaved by miR390.