This study investigated the response features of alpine plant endogenous hormones,total nitrogen on time series and its relationship with growth under enhanced UV-B radiation by using the sample of Vicia unijuga collected from alpine meadow.The supplemental UV-B irradiation was 6.40 kJ/m² per day that simulated 9% stratospheric ozone depletion on clear summer solstice (Gannan,34°55′N,2 900 m),China.The results showed that:(1)The wild legume forage (Vicia unijuga) from alpine region could have stress response to enhanced UV-B radiation at the early stage of treatment.IAA,ZT,GAs,6-BA levels and total nitrogen content increased and were significant higher than that of control at the ten days of treatment.Meanwhile,the plant growth also increased,the plant height and biomass were significant higher than that of control.(2)After 10 days of treatment,the hormones decreased rapidly and reached to the lowest level up to 30 days of treatment.The total nitrogen content also decreased continuously to reach at a significant lower lever than that of control from 10 to 40 days of treatment.The plant growth was inhibited with the reduction of hormones level and the total nitrogen content.(3)ABA has not been detected until the treatment for 40 days.It suggested that the damage to membrane might be occurrence later.The study revealed that the some plant species from alpine region have undergone natural selection and made adaptation to the severe environment for a long term,and developed the special response mechanism to delay the damages to the plant caused by UV-B radiation.