Interspecific relationships in Rehmannia,a quasi-endemic genus in China,are still unresolved.In this study,we used multiple individuals to reconstruct the phylogenetic tree on the basis of chloroplast and nuclear genes.Our results are shown as follows:(1)Monophyly of Rehmannia is strongly supported and R.chingii,a sister or consecutive sister species to R.henryi,is the basic taxon in the genus.R.piasezkii-R.elata and R.glutinosa-R.solanifolia form two highly supported pairs of sister species.(2)Phylogenetic tree constructed by using multiple individual is more informative to uncover the complicate relationships among species.(3)Analysis of ancestral distribution area reconstructed by RASP indicates that there are three times of dispersals and two times of vicariances in the speciation history of Rehmannia,which are closely related to climate changes during Late Miocene and early Pliocene.We also proposed some advices to explore the speciation procedure in the genus.