氯化镁预处理对美人蕉根部质膜H+-ATPase活性与农田废水硝态氮吸收效率的影响
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云南省科技计划(社会事业发展专项2010CA001)


Effects of Magnesium Chloride Pretreatment on the Plasma Membrane H+-ATPase Activity and Nitrate Absorption Efficiency of Canna generalis in Farmland Wastewater
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    摘要:

    以湿地挺水植物七叶红色大花美人蕉(Cana generalis Bailey)幼苗为实验材料,在温室中用150 μmol/L氯化镁溶液预处理12 h,然后置于硝态氮(NO3--N)浓度为10 mg/L的农田废水中处理,考察氯化镁预处理对美人蕉吸收农田废水中硝态氮效率的影响,以及处理期间根部质膜H+-ATPase活性、H+-泵活性、14-3-3蛋白与质膜H+-ATPase相互作用。结果显示:经过150 μmol/L氯化镁溶液预处理12 h后,美人蕉吸收农田废水中硝态氮的效率比对照增加11%;与没有预处理的植株相比,氯化镁预处理美人蕉根中质膜H+-ATPase和H+-泵的活性均显著提高,质膜H+-ATPase和14-3-3蛋白的相互作用显著增强。研究表明,氯化镁能够通过增强质膜H+-ATPase与14-3-3蛋白的相互作用来提高质膜H+-ATPase的活性,从而增加美人蕉对硝态氮的吸收效率。

    Abstract:

    In this study,the seedlings of the wetland emergent plant,Canna generalis Bailey,were used as the experimental materials.Pretreatment of the seedlings with 150 μmol/L MgCl2 was conducted under the greenhouse conditions for 12 h.Subseqently,treatment of the seedlings with farmland wastewater containing 10 mg/L nitrate (NO3--N) was performed to investigate the effects of MgCl2 pretreatment on the nitrate absorption efficiency,the plasma membrane H+-ATPase and H+ pump activities and the interaction of plasma membrane H+-ATPase and 14-3-3 protein of C.generalis in farmland wastewater.The results showed that pretreatment of 150 μmol/L MgCl2 for 12 h increased the nitrate absorption efficiency of C.generalis Bailey in farmland wastewater by 11%.Compared with those in the roots of the plants without MgCl2 pretreatment,both the plasma membrane H+-ATPase and H+ pump activities in C.generalis roots with MgCl2 pretreatment were significantly elevated.MgCl2 pretreatment also enhanced plasma membrane H+-ATPase and 14-3-3 protein interaction in C.generalis roots.These results suggested that MgCl2 pretreatment increased the Plasma membrane H+-ATPase activity by enhancing its interaction with 14-3-3 protein,thereby increasing the nitrate absorption efficiency of C.generalis in farmland wastewater.

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刘 昂,吴文卫,赵 磊,等.氯化镁预处理对美人蕉根部质膜H+-ATPase活性与农田废水硝态氮吸收效率的影响[J].西北植物学报,2014,34(1):128-134

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  • 在线发布日期: 2014-02-13
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