窄叶野碗豆种子的休眠与发芽特性
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国家自然科学青年基金(31001030);国家科技计划(2011BAD17B02)


Seed Dormancy and Germination Characteristics of Vicia angustifolia
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    摘要:

    以青藏高原野豌豆属窄叶野碗豆(Vicia angustifolia)种子为材料,就其休眠类型、发芽对温度与水分的响应以及休眠破除方法进行了研究。结果显示:(1)窄叶野碗豆新鲜种子具有复合休眠特性,而贮藏种子仅具有物理休眠特性。(2)与贮藏种子相比,新鲜种子的发芽温度范围变窄,高温显著抑制其种子的发芽,在25℃条件下新鲜种子的发芽率仅为4%,而贮藏种子可达90%以上。(3)新鲜种子发芽对水分的要求较高,在-0.4 MPa以及10 ℃、15 ℃、20 ℃条件下,新鲜种子的发芽率分别为85%、55%、8%,而贮藏种子的发芽率分别为95%、91%、89%。(4)氟啶酮(FL)和赤霉素(GA3)对贮藏种子的发芽与发芽速率均无明显作用,但可显著提高新鲜种子的发芽速率;与贮藏种子相比,新鲜种子的发芽对脱落酸(ABA)的抑制作用更为敏感;除高浓度多效唑(PA)显著抑制新鲜种子的发芽外,其他浓度的PA 对新鲜种子与贮藏种子均无显著影响。研究表明,脱落酸可能是引致窄叶野豌豆种子生理休眠的主要原因;硫酸及切破种皮处理均可破除贮藏种的物理休眠,其中硫酸处理20 min效果最好。

    Abstract:

    Present study explored seed dormancy type,seed germination response to temperature and water potential,and dormancy break methods of Vicia angustifolia,which extensively distributed in the Qing-Tibetan Plateau.The results showed that:(1)Fresh V.angustifolia seeds exhibited combinational dormancy,but one year stored seeds exhibited physical dormancy (PY) only.(2)Compared to one year stored seeds,fresh seeds had a narrower germination temperature range,and high temperature significantly inhibited its seed germination.Stored seeds germinated to 90% at 25 ℃,but fresh seeds germinated to 4% only.(3)Fresh seeds are more sensitive to water stress compared to stored seeds regardless of temperature.At 10 ℃,15 ℃,20 ℃,stored seeds germinated to 95%,91% and 89% at -0.4 MPa,respectively,and fresh seeds germinated to 85%,55% and 8% only.(4)Fluridon (FL) and gibberellic acid (GA3) had no obvious effect on the germination of stored seeds,but significantly promoted the germination speed of fresh seeds at 20 ℃.Compared with the stored seeds,germination of fresh seeds are more sensitive to ABA inhibition.In addition to the highest concentration of paclobutrazol(PA,100 μmol/L),PA had no significant effect on germination of fresh and stored seeds.This suggests that ABA may be the main reason responsible for physiological dormancy of V.angustifolia seeds.Sulfuric acid scarification and cut treatment both could release seed physical dormancy effectively,and sulfuric acid scarification for 20 min is the optimal way to release physical dormancy of one year stored V.angustifolia seeds.

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张 瑞,李廷山,胡小文,等.窄叶野碗豆种子的休眠与发芽特性[J].西北植物学报,2014,34(1):135-142

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  • 在线发布日期: 2014-02-13
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