Endophytic fungi were isolated from the roots,stems and leaves of Cannabis sativa L.by tissue block method,and degumming strains were screened by transparent circle method,and then identified by morphology and molecular biology methods.The results indicated that:(1)A total of 16 endophytic fungi were isolated from different tissues of the C.sativa,nine of them were from the stems,five then from the leaves and two from the roots (2).(2)For the isolated strains,one of them named DM6 was more effective in decomposing the pectin of the C.sativa,and the transparent circle diameter is 2.49 cm.(3)Morphological identification showed that strain DM6 was non-sporulating and the hyphae was more stout,fewer branches with a distinct septum.Molecular identification indicated that the sequence of strain DM6 was found to share 99% similarity with Phoma aliena(KC311486).Strain DM6 was shown to be close to the genus Phoma.So the isolate was further identified as Phoma sp.