Based on our constructed cDNA full length library,a gene named VpPAP1(GenBank Accession NO.JN624817) encoding plastid lipid-associated protein was cloned and sequenced from leaves of Vitis pseudoreticulata ‘Baihe-35-1’ by RT-PCR(Reverse Transcription PCR).The sequence analysis indicated that the cDNA length was 1 034 bp,flanked by a 5′-untranslated region of 26 bp and a 3′-untranslated region of 63 bp. 945 bp of the open reading frame encodes a polypeptide of 314 amino acid residues,protein molecular weight is 34 169.37 Da and theoretical isoelectric point 7.76.The expression profile of the VpPAP1 was analyzed in V.pseudoreticulata infected Uncinula necator.Realtime-PCR analysis revealed that the VpPAP1 gene was induced by U.necator in leaves,and reached the peak at 24 h,which indicated that it may involve in the resistance to U.necatori.The study will be helpful to study the function of plastid lipid-associated protein on interaction of U.necator and grapevine.