Abstract:Based on analyzed zymograms of leaf peroxidase isozyme (POD),superoxide dismutase (SOD) and bud esterase (EST) by polyacrylamide gel electrophoresis (PAGE),we studied the genetic diversity of 32 Capsicum genus germplasms.The results showed that:(1)A total of 29 kinds of bands were produced,including 13 kinds of 234 POD bands,the proportion of polymorphic loci (PPL) and the range of relative mobility (Rf) were 50.0%~80.0% and 0.05~0.69;8 kinds of 140 SOD bands,PPL was 100.0%,and Rf was 0.38~0.88;8 kinds of 163 EST bands,PPL was 50.0%~87.5%,and Rf was 0.16~0.89.(2)26 polymorphic loci were found,and PPL,total gene frequency (Pt),mean number of alleles (A),Nei’s genetic diversity index (He),Shannon’s information index (SII),average of genetic identity (GI) and genetic distance (GD) were 89.66%,0.578 7,1.896 6,0.272 2,0.415 2,0.736 6 and 0.314 3,respectively;Jaccard genetic similarity coefficients were in range of 0.348~0.905,by which 32 materials were clustered into 4 cultivated populations/species.(3)Genetic diversity within species (HS) of 4 cultivated species was 0.143 6,and genetic diversity among species (DST),gene differentiation coefficients among species (GST),GI and GD were 0.149 4,0.51,0.784 4 and 0.246 6,respectively.(4)In 9 purple germplasms,16 polymorphic loci were found,and PPL,Pt,A,He,SII,GI and GD were 55.17%,0.611 1,1.551 7,0.194 1,0.291 9,0.781 6 and 0.249 4,respectively.In conclusion,high genetic differentiation degree among overall tested materials,or among cultivated species and within species,or among purple materials will provide a reference to selecting parents of purple capsicum crossbreeding and genetic improvement of the green variety.