长药景天对重金属镉胁迫的生理及形态响应
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黑龙江省研究生创新科研资金(YJSCX2012-162HLJ)


Physiological and Morphological Response of Sedum spectabile Boreau under Cadmium Stress
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    摘要:

    以长药景天为实验材料,通过模拟重金属镉(Cd)污染,采用原子吸收光谱法和比色法,确定了Cd在长药景天体内积累最多的部位及长药景天对Cd(NO3)2的耐受浓度阈值;并采用半薄切片法、扫描电镜法及X射线能谱法对100 μmol·L-1 Cd(NO3)2胁迫下长药景天的应答进行分析研究。结果表明:(1)Cd(NO3)2胁迫后,Cd在长药景天根、茎、叶中的分布情况为根>茎>叶。(2)在100 μmol·L-1 Cd(NO3)2浓度处理下,长药景天能够进行正常的生理代谢,且无明显的Cd中毒现象。(3)100 μmol·L-1 Cd(NO3)2处理后,长药景天根表面发生皱缩,根毛明显增多,外表皮与皮层细胞难以区分,薄壁细胞层数明显多于对照组根部皮层细胞层数,排列紧密、不规则,根部横截面积有增大趋势。(4)100 μmol·L-1 Cd(NO3)2处理后,Cd在长药景天根部的分布情况为表皮>皮层>中柱。研究认为,长药景天能够耐受浓度为100 μmol·L-1 Cd(NO3)2的胁迫,在修复土壤Cd污染方面具有潜在的应用价值。

    Abstract:

    With Sedum spectabile Boreau as material,by simulating the heavy metal cadmium(Cd) pollution,we detected the Cd accumulation in plant and the best tolerance on Cd(NO3)2 concentration by using atomic absorption spectrometry and Colorimetric methods.Based on the above research results,Under 100 μmol·L-1 Cd(NO3)2 treatment,morphological response were observed and Cd content in each group of its root were compared by using Semi thin section,scanning electron microscopy(sem) and X-ray spectroscopy.The results show that:(1)After Cd(NO3)2 stress.The distribution of Cd in different organs was uneven,Specifically,root>stem>leaf.(2)Under less than or equal to 100 μmol·L-1 Cd(NO3)2 treatment,S.spectabile Boreau can take days of normal physiological metabolism.When Cd(NO3)2 concentration reaches to 400 μmol·L-1,S.spectabile Boreau apparent Cd poisoning,as severe of membranous of root were badly damaged.(3)The surface of roots of sedum spectalile Boreau occured Shrinkage,Root hair markedly increased,difficult to distinguish outer epidermis and cortex,with more parenchyma cells layers than that of control group,closely packed and inregular.(4)After 100 μmol·L-1 Cd(NO3)2 stress,the distribution of Cd in different tissues was uneven,Specifically,root>stem>leaf.In conclusion,S.spectabile Boreau can tolerate a high concentration of Cd(NO3)2 and has the potential research value for Cd pollution soil repairing.

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焦轶男,朱 宏.长药景天对重金属镉胁迫的生理及形态响应[J].西北植物学报,2014,34(6):1173-1178

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  • 在线发布日期: 2014-07-01
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