In order to study the lignin synthesis related genes of Cryptotaenia japonica Hassk,the gene CjC4H with 1 631 bp,which encoding the cinnamate 4-hydroxylase(C4H) involved into lignin synthesis,was cloned from wild variety C.japonica Hassk of Guizhou Province.C.japonica Hassk was grown under 4 different temperatures(10 ℃,18 ℃,30 ℃ and 38 ℃).The CjC4H genes expression was detected at 0,0.5,1,2,4,8,12 and 24 h to provide reference for C.japonica Hassk suitable growth temperature selection.The ORF(Open Reading Frame) length was 1 521 bp,which encoding 506 amino acids.The molecular weight of the enzyme of CjC4H was 58.13 kD,and the pI was 9.38.Phylogenetic analysis demonstrated that the enzyme of C4H relative conservative in different plant species.The CjC4H genes expression under different temperature detected by real time quantitative PCR,the results showed that the CjC4H gene expression under high temperature of 38 ℃ 4 h was much higher than that of other temperature treatments,and the expression of CjC4H increased significantly at 24 h under 10 ℃ and 18 ℃ low temperature treatments.