3个马铃薯杂种优良株系的核型及SSR分析
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国家科技支撑计划(2012BAD02B05);内蒙古自然科学基金重大项目(2013ZD03);内蒙古马铃薯产业发展重大技术集成创新与示范项目(20131706);国家现代农业产业技术体系建设专项资金资助(CARS-10-P17)


Karyotype and SSR Analysis of 3 Potato Hybrid Lines
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    摘要:

    为明确‘费乌瑞它’ב陇薯3号’杂种F1优良无性株系A1和A3及‘费乌瑞它’×J07-6杂种F1优良无性株系C2在染色体及DNA水平上的遗传差异,利用根尖染色体制片技术和SSR分子标记技术,对这3个优良株系的核型及SSR指纹特征进行分析。结果表明:(1)株系A1、A3和C2均为四倍体(2n=4x=48),其核型类型分别为2B、1B和1A;株系A1和A3核型公式均为2n=4x=48=36m+12sm,C2核型公式为2n=4x=48=48m。(2)利用筛选出的10对SSR适宜引物PCR扩增得到50个多态性位点,多态性位点百分率为79.37%。(3)建立了3个马铃薯杂种优良株系及其亲本的SSR指纹图,并以遗传距离(GD值)0.45为基准,将6个材料分为3类:母本‘费乌瑞它’、株系A1和父本‘陇薯3号’聚为一类,株系A3和C2聚为另一类,父本J07-6单独列为一类。

    Abstract:

    To identify the genetic differences among hybrid lines A1 and A3 derived from a cross of ‘Favorita’בLongshu 3’,and hybrid line C2 from a cross of ‘Favorita’×J07-6 in chromosome and DNA level,we analyzed their karyotype and SSR fingerprint characteristics using root tip chromosome technology and SSR molecular marker technology.The result showed that:(1)Hybrid line A1,A3 and C2 were tetraploid(2n=4x=48),with karyotype type of 2B,1B and 1A,respectively.The karyotype formula of A1 and A3 were 2n=4x=48=36m+12sm and 2n=4x=48=48m,respectively.(2)A total of 50 polymorphic loci were amplified using the 10 suitable SSR primer pairs,accounting for 79.37% of all the amplified loci.(3)The SSR fingerprints of the 3 hybrid lines and their parents were established,which provided a basis for the identification of the hybrid lines and their parents at DNA level.The materials were classified into three categories based on genetic distance(GD) value of 0.45:mother favorite,hybrid line A1 and father ‘Longshu 3’ as a category,hybrid line A3 and C2 as a category,and father J07-6 as a category.

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张自强,于肖夏,鞠天华,等.3个马铃薯杂种优良株系的核型及SSR分析[J].西北植物学报,2014,34(7):1318-1324

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  • 在线发布日期: 2014-08-15
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