Abstract:To identify the genetic differences among hybrid lines A1 and A3 derived from a cross of ‘Favorita’בLongshu 3’,and hybrid line C2 from a cross of ‘Favorita’×J07-6 in chromosome and DNA level,we analyzed their karyotype and SSR fingerprint characteristics using root tip chromosome technology and SSR molecular marker technology.The result showed that:(1)Hybrid line A1,A3 and C2 were tetraploid(2n=4x=48),with karyotype type of 2B,1B and 1A,respectively.The karyotype formula of A1 and A3 were 2n=4x=48=36m+12sm and 2n=4x=48=48m,respectively.(2)A total of 50 polymorphic loci were amplified using the 10 suitable SSR primer pairs,accounting for 79.37% of all the amplified loci.(3)The SSR fingerprints of the 3 hybrid lines and their parents were established,which provided a basis for the identification of the hybrid lines and their parents at DNA level.The materials were classified into three categories based on genetic distance(GD) value of 0.45:mother favorite,hybrid line A1 and father ‘Longshu 3’ as a category,hybrid line A3 and C2 as a category,and father J07-6 as a category.