宝兴百合与匍茎百合遗传多样性的ISSR分析
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国家自然科学基金(31100161,31270241);国家基础条件平台教学标本资源共享平台(http://mnh.scu.edu.cn/)


Genetic Diversity of Lilium duchartrei and Lilium lankongense Revealed by ISSR Markers
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    摘要:

    利用5条ISSR引物对宝兴百合(Lilium duchartrei)9个居群和匍茎百合(Lilium lankongense)13个居群的遗传多样性进行了初步检测。结果表明:(1)宝兴百合在物种水平上多态位百分率(PPB)为97.26%,Nei’s基因多样度(H)为0.309 8,Shannon’s多样性信息指数(Hsp)为0.469 4;匍茎百合在物种水平上多态位百分率(PPB)为100%,Nei’s基因多样度(H)为0.3390,Shannon’s多样性信息指数(Hsp)为0.503 0,均略高于宝兴百合。(2)宝兴百合与匍茎百合的遗传多样性在居群水平上相对较低;宝兴百合和匍茎百合居群间遗传分化系数(Gst)分别为0.642 5和0.563 7,表明2个物种居群间的遗传分化大于居群内的遗传分化。(3)经Mantel检测,两种居群间的遗传距离与地理距离均不存在显著的相关性(宝兴百合r=0.263 7,P=0.844 0;匍茎百合r=0.104 2,P=0.695 0);宝兴百合与匍茎百合的遗传多样性及遗传分化现状可能是两者的生活史特性、地理隔离等作用的结果。(4)UPGMA聚类结果显示,宝兴百合与匍茎百合在分子水平上出现了明显分化,支持二者是独立的物种。

    Abstract:

    The genetic diversity of 9 populations of Lilium duchartrei and 13 populations of Lilium lankongense was analyzed by inter-simple sequence repeat makers (ISSR).(1)For L.duchartrei,the percentage of polymorphic loci(PPB) at the species level,Nei’s gene diversity(H) and Shannon’s information index(Hsp) was found to be 97.26%,0.309 8 and 0.469 4,respectively,whereas for L.lankongense,the three indices were found to be 100%,0.339 0 and 0.503 0,respectively,all higher than that of L.duchartrei’s.(2)There existed little lower genetic variation at population level.The coefficient of genetic differentiation among populations(Gst) was 0.642 5 for L.duchartrei and 0.563 7 for L.lankongense,suggesting that for both species there is a high genetic differentiation among populations.(3)Both of L.duchartrei and L.lankongense,the Mantel test showed no significant correlation between genetic and geographic distances.The genetic diversities and the current genetic differentiation of L.duchartrei and L.lankongense might have resulted from combined effects,including those of life history traits and geographical isolation.(4)UPGMA analysis show significant genetic differentiation between L.duchartrei and L.lankongense at molecular level,supporting the view that they are separate species.

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沈呈娟,周颂东,何兴金.宝兴百合与匍茎百合遗传多样性的ISSR分析[J].西北植物学报,2014,34(7):1331-1338

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  • 在线发布日期: 2014-08-15
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