Abstract:To determine the effect of different concentration methanol solution on seed germination and seedling growth of Brassica napus L.,we chose two main varieties ‘Yuyou 2’ and ‘Qinyou 2’ as materials to be cultured by water or 10—2 000 mmol·L-1 methanol solutions.Seed germination,seedling growth properties and its related physiochemical indices were analyzed.The results indicated that:(1)10—1 000 mmol·L-1 methanol solution obviously enhanced seed germination parameters(including germination rate,germination potential,germination index and vigor index) and seedling growth parameters(including shoots length,roots length,fresh weight and root/shoot ratio) of B.napus L.than that of the control,but the these parameters significantly reduced at 2 000 mmol·L-1.The two varieties showed the same trend under methanol solution treatment,but there were also slight differences in the two varieties,i.e.‘Qinyou 2’ was affected lower than ‘Yuyou 2’.(2)With increasing concentrations of methanol,B.napus L.seed germination index and seedling growth index increased at first,but decreased later.With 100 mmol·L-1 methanol solution treatment,seed germination parameters and seedling growth parameters of B.napus L.reached the highest value.With higher concentration,the promotion was weaken or disappear,even dampen.(3)With increasing concentrations of methanol,B.napus L.seedling leaves chlorophyll content also showed an increasing trend and decreased at late.The MDA and hydrogen peroxide contents at first fell after the raised of the opposite trend in the same period.The results showed that the appropriate concentration of methanol solution could promote seed germination and seedling growth of B.napus L..