Abstract:Orthoploidy Saccharum spontaneum L.is an important wild parent and gene pool related stress tolerance of modern sugarcane varieties,in order to understand their genetic background of resistant genes,we screened 50 clones of decaploid S.spontaneum L.with 63 sugarcane commonly used parents and 8 original species as control in genetic diversity by using these functional markers derived from DREB,AQP,HSP70,WRKY1 and arbitrary primers,of which are related to drought and high temperature tolerance.The results showed that:(1)119 bands were gained,110 of which belong to polymorphic ones,and the mean percentage of polymorphic bands and polymorphism information content reached to 92.44% and 92.53% respectively.The polymorphic level of decaploid S.spontaneum L.in the functional markers related drought tolerance was the richest;however the commonly used parents exhibited the most diversity in functional markers related high temperature tolerance.(2)In terms of Jaccard coefficient,decaploid S.spontaneum L.showed low mean value(0.541) and big distribution range(0.347-0.800) in the functional markers of drought tolerance,and most focused on the interval region of 0.400-0.600;for functional markers of high temperature tolerance,the biggest distribution range(0.238-1.000) appeared in commonly used parents,whereas the lowest value of coefficient(0.481) was found in the original materials mainly distribute with the interval region of 0.400-0.600.(3)By using UPGMA cluster method,all accessions could be divided into two big groups,one was consisted of decaploid S.spontaneum L.accessions,which exhibited evident difference from others;the other was composed by commonly used parents and original species.Overall,our results proofed that the decaploid S.spontaneum L.accessions have big genetic differences in functional markers of DBF,Aqua,SCB174 and SCB190 from commonly used parents and original species,and possess more abundant genetic variances in functional markers of drought tolerance.So in future,decaploid S.spontaneum L.resources should be utilized in improving stress tolerance of sugarcane varieties,especially in the impact of drought tolerance.