In this study,the gene GPX(glutathione peroxidase) was cloned by RACE from the seeds of ‘Hongyushizi’,a cultivar of pomegranate.The expression of PgGPX in different organs of ‘Hongyushizi’ and its leaves under salt stress were analyzed by Real-time PCR.The results showed that the length of PgGPX was 872 bp and its ORF was 504 bp which encoding 168 amino acids.Bioinformatics analysis showed PgGPX possessed a typical conserved domain of GSH-Peroxidase and had high consistency with Theobroma cacao,Litchi chinensis,Ricinus communis and Zea mays as 90.30%,87.40%,86.80% and 86.20%,respectively.The real-time PCR results showed the expression of PgGPX in leaf,petal and aril were all detected and its expression had tissue specificity.The expression levels of PgGPX increased obviously under salt stress indicated that it may play an important role in stress reaction.