Abstract:With the floral organs of ‘Eyeliner’ as explants,we established a rapid floral organ propagation technology via adventitious buds induction and callus redifferentiation.The results showed that:the contamination rate was 0 and the inducing abilities of different parts of floral organ were different,their sequence,from easy to hard,was pedicel,filament,ovary and style.Callus and adventitious sprouts was efficiently induced from in vitro filament as well as pedicel on MS medium appended with 1.0 mg·L-1 6-BA and 0.5 mg·L-1 NAA.The appropriate medium for adventitious buds multiplication was MS+2.0 mg·L-1 6-BA+0.2 mg·L-1 NAA,and MS supplemented with 1.5 mg·L-1 6-BA+0.2 mg·L-1 NAA was the optimum medium for callus differentiation.The appropriate medium for in vitro root and bulb formations was MS+45 g·L-1 sucrose.The optimum way of acclimatization as follows:placing the sealed plantlets in natural environment for 3 days,and then exposing them for 2 days.The highest survival rate reached when the bulb size was 3.15 cm.The suitable transplantation matrix was peat∶vermiculite∶perlite=2∶1∶1.