Abstract:With different origins of Dendrobium officinale fresh stem produced from 12 different areas segments as material,we performed gradient elution,with Ultimate XB C18 (4.6 mm×250 mm,5 μm) acetonitrile-0.1% phosphoric acid solution as mobile phase gradient elution,velocity of 1.0 mL·min-1,detection wavelength of 270 nm,column temperature 30 ℃.Through Chinese medicine chromatographic fingerprint similarity evaluation system (national pharmacopoeia committee) similarity analysis and establish the 12 different areas fingerprint,at the same time by microwave digestion processing materials,using ICP-AES atomic absorption spectrometry determination of different origin of cadmium,copper,lead and mercury contents,so as to compare the different origin of quality,to provide theoretical basis for establishing quality standard.The results show:(1)A total of 23 different producing area features was established.The similarity of different origin of D.officinale was 0.918-0.987,which shows differences,but there was no difference in the chemical composition and chemical contents;(2)Through clustering analysis,D.officinale of 12 different regions can be divided into four categories:Anhui Huoshan,Jiangsu Nanjing,Yunnan Shiping,Yunnan Yuxi,Yunnan Simao,as a category,Yunnan Wenshan,Zhejiang Xianju as a category,Yunnan Honghe,Guangxi Tian’e,Dabie Mountain in Anhui and Fujian Ninghua as a class,Zhejiang Tiantai for a class;(3)Including the copper contents from Yuxi of Yunnan,cadmium contents from Fujian Ninghua,Jiangsu Nanjing,exceed 26.25%,27.33% and 122.30%,respectively.Other orgin of copper,cadmium,lead,mercury levels in countries.Our aim is to provide theoretical basis for D.officinale medicinal materials quality control.