盐穗木HcDmc1基因的克隆和表达分析
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国家“973”计划前期研究专项(2012CB722204);新疆重点实验室专项资金(2014KL001)


Clone and Expression Analysis of HcDmc1 Gene from Halostachys caspica
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    摘要:

    根据盐穗木盐胁迫下响应的转录组测序结果,克隆获得盐穗木DNA损伤修复基因的cDNA序列,其开放阅读框1 035 bp,编码344个氨基酸,命名为HcDmc1。保守结构域分析显示,该基因编码的蛋白具有RecA 蛋白家族典型的保守结构域;统进化树分析显示HcDmc1为独立的分支;亚细胞定位于细胞质,为无信号肽、不跨膜的稳定亲水性蛋白。实时荧光定量PCR分析表明,盐穗木在100 mmol/L NaCl胁迫7 d后,同化枝中HcDmc1基因表达迅速上调并达到最大值,约为对照组的6.58倍;在700 mmol/L NaCl胁迫14 d后根中HcDmc1基因表达最高,约为对照的1.79倍。研究表明,HcDmc1基因表达受盐胁迫诱导。

    Abstract:

    According to the transcriptome of Halostachys caspica under salt stress,we isolated an cDNA fragment from H.caspica.The obtained cDNA was named as disrupted meiotic cDNAof H.caspica.Sequence analysis indicated that HcDmc1 contains an open reading frame (ORF) of 1 035 bp,which encodes 344 amino acids.Conserved domain analysis showed that HcDmc1 has a conserved domain of RecA protein family.Phylogenetic tree analysis indicated that HcDmc1 is an independent branch,which is an stable hydrophilic proteins sub-cellularly cytoplasm.Real time quantitative PCR results showed that the expression of HcDmc1 gene in branch after 100 mmol/L NaCl salt stress for 7d was rapidly up-regulated and reached the highest after 700 mmol/L,which was 6.58-fold of the control.While in roots the expression of HcDmc1 reached the highest level at 14 d and about 1.79-fold of the control.Base on the experimental results,the expression of HcDmc1 gene could be induced by salt stress.

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杜 驰,张富春.盐穗木HcDmc1基因的克隆和表达分析[J].西北植物学报,2015,35(2):220-226

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  • 在线发布日期: 2015-03-21
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