苦荞锌指蛋白基因FtLSD1的克隆及其对非生物胁迫的应答
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

四川省教育厅青年基金(13ZB0294)


Cloning and Expression Analysis of Zinc Finger Protein Gene FtLSD1 in Fagopyrum tataricum under Abiotic Stress
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    根据苦荞(Fagopyrum tataricum)花期转录组数据,分别以苦荞DNA和cDNA为模板,克隆得到1个苦荞C2C2型锌指蛋白基因FtLSD1(GenBank登录号KP252134)的DNA序列和cDNA序列,采用实时荧光定量PCR方法,研究了FtLSD1基因在非生物胁迫下的表达模式。结果显示:苦荞FtLSD1基因DNA全长2 427 bp,由6个外显子和5个内含子构成,符合GU-AG剪切原则;cDNA序列包含一个528 bp开放阅读框,编码175个氨基酸,具有LSD1家族的典型结构域;UV-B照射和水杨酸处理均能使FtLSD1基因的表达量上升,且UV-B处理在6 h达到最大,为0 h(CK)的3.84倍;水杨酸处理于10 h达到最大,为0 h(CK)的3.44倍,而4 ℃冷胁迫下该基因表达量保持稳定。推测该基因可能参与苦荞抗UV-B和高浓度水杨酸等非生物胁迫的应答反应,为苦荞的抗逆性研究提供新的视角。

    Abstract:

    According to transcriptome data of Fagopyrum tataricum at flowering,using PCR and RT-PCR techniques,the DNA and full-length cDNA sequences of FtLSD1 gene (GenBank accession number:KP252134) were amplified from F.tataricum.The obtained sequences were analyzed by bioinformatics software,and the expression FtLSD1 gene were analysed by qPCR under UV-B,SA and 4 ℃ cold stress.The results showed that the DNA sequence of FtLSD1 gene was 2 427 bp,of which consisted 6 exons and 5 introns,in line with the principle of GU-AG splicing,and the cDNA of FtLSD1 contained a 528 bp ORF.The UV-B radiation and 2 mmol/L salicylic acid could lead to a significant increase in the expression of the FtLSD1 gene,while 4 ℃ cold stress remained stable expression levels of the gene.The results expected to lay a foundation for study the stress resistance in F.tataricum.

    参考文献
    相似文献
    引证文献
引用本文

高 飞,姚攀锋,雒晓鹏,等.苦荞锌指蛋白基因FtLSD1的克隆及其对非生物胁迫的应答[J].西北植物学报,2015,35(4):669-673

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2015-05-08
  • 出版日期:

微信公众号二维码

手机版网站二维码