Abstract:The pollen morphology of Camellia magniflora was investigated under scanning electronic microscope(SEM),Fresh pollen of C.magniflora was took as materials to test the pollen viability by in vitro.Effects of different store methods on the pollen viability,SOD,POD and CAT were also investigated.The results show that (1)The polar face is a equilateral triangle,the germinal aperture is wider on the polar.The exine ornamentation were mainly perforate,sulciform.The average length of polar axis is 71.05 μm,which show C.magniflora should occupy special taxonomic status in the genus Camellia.(2)The best combination for pollen germination is 150 g·L-1 sucrose,20 mg·L-1 boric acid,100 mg·L-1 KNO3,50 mg·L-1 MgSO4,300 mg·L-1 Ca(NO3)2.The germination rate reached 68.10%.(3)-80 ℃ sealed storage is the best storage condition to keep the vitality of pollen,the germination rate of pollen reached 39.40% after 360 d storage.(4)The three kinds of enzyme activities were higher,Antioxidant,aging ability were also higher in -20 ℃ and -80 ℃ storage.The germination proportion of pollen decreased significantly when the activities of SOD,POD and CAT were around the highest levels;(5)The three enzymes had different responses to the storage temperature.SOD was a responding protection enzymes at normal temperature,POD was a responding protection enzymes at 4 ℃ and -20 ℃,while CAT was a responding protection enzymes at -80 ℃.The effect of three kinds of enzymes on pollen germination is CAT>SOD>POD.