羽衣甘蓝对镉的耐性和富集特征研究
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国家自然科学基金(41101298)


Cadmium Tolerance and Accumulation Characteristics of Brassica oleracea L.var.acephala DC.
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    摘要:

    以观赏绿化植物羽衣甘蓝(Brassica oleracea L.var.acephala DC.)为试验材料,采用盆栽试验,研究其对镉的耐性和富集特征,探讨羽衣甘蓝对镉污染土壤生物修复的可行性。结果显示:(1)随着镉处理浓度的增加,羽衣甘蓝干重呈先升高后降低的趋势,20~80 mg·kg-1镉处理能促进植株的生长,100~120 mg·kg-1镉处理虽然显著抑制了根系的生长,但是促进了地上部的生长,整体而言对全株生长无明显影响,表明羽衣甘蓝对镉有较强的耐性,且地上部的耐性高于根系。(2)所有镉处理下,羽衣甘蓝叶片的SOD、POD和APX活性较高,MDA含量和电解质渗漏率与对照无差异;而镉处理浓度高于80 mg·kg-1时,根系的SOD、CAT和APX活性显著降低,MDA含量和电解质渗漏率显著升高,引起严重的膜脂过氧化伤害,这可能是羽衣甘蓝地上部的镉耐性高于根系的原因之一。(3)羽衣甘蓝地上部的镉含量高于根系,且随着镉处理浓度的增加均逐渐增加,在镉处理浓度为120 mg·kg-1时达到最大值,分别为50.15 mg·kg-1(根系)和52.01 mg·kg-1(地上部);植株对镉的转运系数大于1,地上部镉富集量高于根系,地上部最大富集量为每株343.19 μg。研究表明,羽衣甘蓝对镉有很强的耐性和富集、转运能力,是一种良好的修复镉污染土壤的观赏绿化植物资源。

    Abstract:

    The ornamental plant,Brassica oleracea L.var.acephala DC.was selected to study characteristics of tolerance and accumulation for cadmium (Cd),aiming to explore its feasibility in phytoremediation.The results showed that:(1)the dry weight of B.oleracea L.var.acephala DC.increased at first and then decreased with increased Cd concentrations,20-80 mg·kg-1 Cd treatment could promote the growth of B.oleracea.Higher Cd concentration at 100-120 mg·kg-1 inhibited roots growth significantly,while promoted growth of shoots,and there was no distinctive difference in whole plant,showing that B.oleracea L.var.acephala DC.had strong capability in tolerance,and the tolerance in shoots was higher than in roots.(2)B.oleracea L.var.acephala DC.has higher activities of antioxidant enzymes such as SOD,POD,APX and lower contents of MDA and electrolyte leakage in leaves.But when the Cd concentration exceed 80 mg·kg-1,the activities of antioxidant enzymes such as SOD,CAT and APX in roots were decreased,and MDA contents and electrolyte leakage increased significantly,induced severe membrane lipid peroxidation damage,this may be one of the reason of higher tolerance for Cd in shoots than in roots.(3)Cd contents in shoots was higher than in roots,and Cd content in plants increased gradually with the increase of Cd concentration,and reached the maximum at 120 mg·kg-1 Cd treatment,and the highest Cd contents in roots,shoots were 50.15 mg·kg-1 and 52.01 mg·kg-1,respectively.The translocation factor were above 1,Cd accumulation amount in shoots was higher than in roots,and the highest Cd accumulation amount of shoots was 343.19 μg·plant-1.These results indicated that B.oleracea L.var.acephala DC.had strong capability in tolerance,accumulation and translocation of Cd,and it is a preferable phytoremediation germplasm resources in ornamental plant for Cd polluted soil.

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贾永霞,李 弦,张长峰,等.羽衣甘蓝对镉的耐性和富集特征研究[J].西北植物学报,2015,35(5):971-977

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  • 在线发布日期: 2015-06-09
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