干旱胁迫下棉花幼苗转录因子BES1/BZR1对外源油菜素内酯的响应表达特征
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国家自然科学基金-新疆联合基金重点项目(U1303282)


Expression Characteristics of Transcription Factor BES1/BRI1 of Cotton Seedling in Response to Brassinosteroid under Drought Stress
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    摘要:

    植物激素油菜素内酯(Brassinosteroid,BR)具有提高植物抗旱性的作用,该研究探讨干旱胁迫下外源喷施BR对棉花干旱胁迫响应基因表达的影响。采用PCR方法从棉花‘新陆早17号’幼苗克隆获得1个干旱胁迫响应转录因子基因,命名为GhBES1/BZR1(GenBank登录号KP272000)。序列分析表明GhBES1/BZR1基因开放阅读框为960 bp,编码319个氨基酸,理论分子量为34.3 kD,理论等电点为8.95。保守结构域分析显示,该基因编码的蛋白具有一个DUF822保守结构域。利用2.5% PEG-6000对棉花‘新陆早17号’幼苗进行干旱胁迫处理24 h,再分别喷施水、BR和Z(BR抑制剂),qRT-PCR分析结果表明,PEG-6000干旱胁迫下用BR处理3 h后,GhBES1/BZR1基因表达量明显提高,当BR处理6和12 h时,GhBES1/BZR1基因的表达量较3 h时明显下降。研究认为,在干旱胁迫下对棉花喷洒BR,GhBES1/BZR1基因能够快速表达以响应干旱胁迫,外源喷施BR有助于提高棉花的抗旱能力。

    Abstract:

    Plant hormone brassinosteroid (BR) can improve the drought resistance of plant.This study explores the effects of spraying BR on the expression of transcription factor BES1/BRI1 in cotton seedling under drought stress.It is helpful for elucidating the mechanism of BR improving the drought resistance of cotton.The BES1/BZR1 gene was cloned by using PCR technique from the seedling of cotton‘XLZ17’(Gossypium hirsutum) and named as GhBES1/BZR1 (GenBank accession number KP272000).Sequence analysis indicated that GhBES1/BZR1 contains an open reading frame (ORF) of 960 bp,which encodes 319 amino acids included a conserved domain DUF822.The calculated molecular weight is 34.3 kD and the theoretical isoelectric point is 8.95.After 2.5% PEG-6000 treatment for 24 h,water,BR (brassinosteroids) and Z (BR inhibitor) were sprayed on the seedlings of cotton‘XLZ17’,respectively.The effects of spraying BR on the expression of GhBES1/BZR1 were analyzed by the real time quantitative PCR(qRT-PCR).The results showed that the expression levels of GhBES1/BZR1 were greatly increased after BR spraying within 3 h,and then the expression levels of GhBES1/BZR1 were significantly decreased after 6 and 12 h.The results demonstrated that spraying BR quickly up-regulated the expression of GhBES1/BZR1 under drought stress,which might be contributed drought resistance of cotton.

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安汶铠,常丹,张富春.干旱胁迫下棉花幼苗转录因子BES1/BZR1对外源油菜素内酯的响应表达特征[J].西北植物学报,2015,35(7):1311-1316

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  • 在线发布日期: 2015-08-29
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