1个新巨胚等位基因的鉴定与表达分析及其性状考察
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上海市科委项目(063919141)


Molecular Characterization,Phenotype Investigation and Expression Analysis of a New Giant Embryo Allele
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    摘要:

    该研究以‘超2-10’水稻和‘上师大5号’巨胚水稻为材料,分析了2种水稻的胚重、胚与糙米质量比、巨胚性状的遗传,克隆和分析了其巨胚基因(GE)序列,并对巨胚基因进行了表达分析。结果显示:(1)‘上师大5号’的胚重、胚与糙米质量比均极显著大于‘超2-10’,且‘超2-10’的胚重接近或大于一些已报道的巨胚水稻;(2)‘上师大5号’巨胚表型由1对隐性基因控制,并与韩国巨胚稻‘M-2-565-11-3-B(ge)’属于相同的等位基因突变;(3)‘超2-10’、‘上师大5号’和‘M-2-565-11-3-B(ge)’ 3种水稻各自具有1个新的GE等位基因,它们的突变位点分别位于启动子、启动子及编码链、编码链;(4)‘上师大5号’启动子及编码链都有突变的GE等位基因(gec),在开花后4~7 d的颖果中表达量持续增加,完全不同于只有编码链突变的GE等位基因呈持续下降的表达模式。该研究首次报道了突变在启动子、突变同时发生在启动子及编码链2种新型巨胚等位基因。

    Abstract:

    In this paper,we used normal embryo rice ‘Chao2-10’ and giant embryo rice ‘Shangshida No.5’ as materials.The embryo weight and mass ratio of embryo to brown rice of ‘Shangshida No.5’ were compared with those of ‘Chao2-10’.Then we did the genetic analysis of giant embryo phenotype.Giant embryo gene (GE) of ‘Shangshida No.5’,‘Chao2-10’ and Korea giant embryo rice ‘M-2-565-11-3-B(ge)’ were cloned to analyse their sequence.The expression levels of GE gene in ‘Shangshida No.5’,‘Chao2-10’ were analysed by Real time-PCR with the ‘Nipponbare’ as a control.The results were as follows:(1) Both embryo weight and mass ratio of embryo of brown rice ‘Shangshida No.5’ were significantly larger than these of ‘Chao2-10’ (P<0.01).However,embryo weight of the ‘Chao2-10’ is close to or more than some of the giant embryo rice cultivars reported previously;(2) Giant embryo phenotype of ‘Shangshida No.5’ is controlled by a single recessive gene,which is same to ‘M-2-565-11-3-B(ge)’;(3) GE gene in these three cultivars is also a new GE allele:‘Chao2-10’ has a mutation (gep) in the promoter;‘Shangshida No.5’ has mutations (gec) both in the promoter and CDS;‘M-2-565-11-3-B(ge)’ only has a mutation (geh) in CDS;(4) The expression pattern of the gec in young caryopses was different from other GE alleles reported,which increase steadily from 4 day to 7 day after flowering.This paper first reported two new GE alleles,one is mutated in the promoter,and the other has mutations in both promoter and CDS.

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平宝哲,时亚琼,谢米雪,等.1个新巨胚等位基因的鉴定与表达分析及其性状考察[J].西北植物学报,2015,35(8):1518-1523

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  • 在线发布日期: 2015-08-29
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