Abstract:The F2 population was derived by crossing two diverse Cucurbita maxima lines ‘98-2-351’ and ‘06820-1’.Investigation and statistical analyses were conducted on rind and flesh color of mature fruits for parental plants and each generation.The results demonstrated that the orange and grey rind plants in F2 population were fitted for the ratio of 3∶1,indicating that the grey rind was controlled by a single recessive gene;similarly,the yellow and white flesh plants in F2 population were also fitted for the ratio of 3∶1,showing that the white flesh was controlled by a single recessive gene.With bulked segregation analysis and recessive-class approaches,two SSR markers PU078072 and PU013839 were detected to be tightly linked to the locus CmRc at a distance 5.9 and 14.5 cM,respectively.Meanwhile,one SSR marker PU132712 was founded to be closely linked to the locus CmFc at a distance 6.7 cM.This study laid the foundation for further screening the molecular markers closer linked to rind and flesh color genes of C.maxima and locating them accurately.