岩高兰分泌组织显微结构特征及其挥发性成分分析
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科技部基础性工作专项(2014FY210400)


Empetrum nigrum Secretory Tissue Microscopic Structure Features and Volatile Components Analysis
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    摘要:

    采用光学显微镜、扫描电镜对岩高兰茎、叶表面、断面及其分泌组织特征进行观察,采用水蒸汽蒸馏法、溶剂提取取法-水蒸汽蒸馏法、溶剂提取法对岩高兰挥发性成分进行提取,并通过GC-MS法对挥发性成分进行对比分析。结果表明:(1)岩高兰的分泌组织有腺毛和分泌细胞;叶表面有头状腺毛,由1个基细胞、1~3个柄细胞、1~4个头部细胞组成;茎髓中含有油细胞,呈黄棕色。(2)水蒸气蒸馏法和溶剂提取法-水蒸气蒸馏法提取率较低,但气味与原植物相近,共有成分10种,多带有芳香气味;溶剂提取法得率较高,但杂质较多。(3)3种方法所得挥发油中有顺-α,α-5-三甲基-5-乙烯基四氢化呋喃-2-甲醇和植酮2个共有成分;从茎和叶挥发性成分中分别鉴定出27和32种化合物,共有成分15种,含量较高的有三十四烷、二十九烷、γ-毕澄茄烯等。研究认为,分泌组织与岩高兰香气物质产生密切相关,其分泌物的组成和含量对香气有重要影响,正是这些有芳香气味的化学成分的有机结合以及自身生长的环境因素才形成了岩高兰的特征香味,此研究结果可为这一香料资源的研究与开发利用奠定基础。

    Abstract:

    By optical microscopy and scanning electron microscopy,we examined the microscopic structure features of secretory tissue in the leaves and stem surface and transverse section of Empetrum nigrum,using steam distillation,solvent extraction-steam distillation and solvent extraction extracting the volatile components of E.nigrum,by GC-MS method we analyzed the volatile components.The results show:(1)the glandular trichomes and secretory cells are E.nigrum secreting tissue.Oil cells in the pith of stem was yellow-brown color,glandular trichome in the leaf surface,it composed of 1 basal cell,a stalk cell 1-3,1-4 head cells.(2)The steam distillation and solvent extraction-steam distillation extraction rate was lower,but the smell similar to the original plants.There are a total of 10 ingredients,more aromatic odor,solvent extraction method extraction rate was higher,but a lot of impurities.(3)Three different methods contain volatile oil composition of compounds:2-furanmethanol,5-ethenyltetrahydro-π 5- trimethyl-,cis and 2-pentadecanone,6,10,14-trimethyl.27 and 32 compounds were identified from parts of stem and leaf of E.nigrum.There were 15 common components in the identified ones,mainly including tetratriacontane,nonacosane and naphthalene,1,2,3,4,4a,5,6,8a-octahydro-7-methyl-4-methylene-1-(1-methylethyl)-,(1π4aπ8aπ)- and so on.Studies suggest that secretory tissue is closely related to E.nigrum aroma matter creation.The composition and content of aroma discharge has important influence.It is the organic combination of these aromatic chemical composition and its growth and environmental factors to the formation of the characteristic flavor of the E.nigrum.The results of this study can provide reference for the research of development and utilization of this spice resource.

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孔凡丽,包海鹰.岩高兰分泌组织显微结构特征及其挥发性成分分析[J].西北植物学报,2015,35(8):1587-1596

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  • 在线发布日期: 2015-08-29
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