The cDNA and DNA sequences of β-1,3-glucanase genes (Mugsp1.2-Mugsp5) were cloned from the leaves of the wild banana（Musa spp.） in Sanming City using RT-PCR and RACE methods.Sequences and bioinformatics analysis showed that the ORF sequences of Mugsp1.2,Mugsp2,Mugsp3,Mugsp4 and Mugsp5 were 1 020,1 047,999,1 023 and 960 bp,respectively,which encoded 339,348,332,340 and 319 amino acids,respectively.Mugsp1.2,Mugsp2 and Mugsp4 contained both N-terminal and C-terminal(CTPP) signal peptide,suggesting they belong to Ⅰclass of β-1,3-glucanase,while Mugsp3 contained N-terminal without CTPP signal peptide,and Mugsp5 contained neither N-terminal nor CTPP signal peptide.We constructed the expression vectors of Mugsp1.2,Mugsp2,Mugsp4 and expressed the genes in onion epidermal cells.The results of subcellular localization showed that Mugsp1.2,Mugsp2,Mugsp4 were expressed on plasma membranes and cytoplasm at room temperature,respectively,while all Mugsp1.2,Mugsp2 and Mugsp4 could transfer to cell nucleus under 8 ℃.The expressions of β-1,3-glucanase genes under diffierent tempertaures were dectected by qRT-PCR.The results revealed that all β-1,3-glucanase genes responded to cold stress,but their expression levels were differed from each other.SA treatment under low temperature would delayed the expression of β-1,3-glucanase genes.