硫酸铈对硝酸钙胁迫下番茄叶片抗氧化酶活性及光合性能的影响
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安徽省现代农业产业技术体系专项-蔬菜产业技术体系( AHCYTX-13);安徽科技学院稳定人才基金(ZRC2013348) 。


Effects of Cerium Sulfate Foliar Spray on Antioxidant Activities and Photosynthesis of Tomato Seedling Leaves under Calcium Nitrate Stress
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    摘要:

    以番茄品种‘合作908’幼苗为材料,研究了80 mmol/L硝酸钙胁迫下叶面喷施10 mg/L硫酸铈对番茄幼苗生长、叶片抗氧化酶活性及光合性能的缓解效应。结果显示:硝酸钙胁迫导致番茄幼苗的株高、茎粗、鲜重、地上和地下部干重显著降低,叶片SOD、POD、CAT活性减弱,净光合速率、气孔导度、蒸腾速率以及胞间CO2浓度均显著下降,而MDA含量和质膜透性显著升高;与硝酸钙胁迫处理相比,叶面喷施硫酸铈处理对硝酸钙胁迫下的番茄幼苗株高、茎粗、鲜重和干重等营养生长具有不同程度的促进作用,同时显著提高叶片SOD、POD和CAT活性,有效降低叶片MDA含量和细胞膜透性,并且显著提高了叶片净光合速率、气孔导度、蒸腾速率。研究表明,叶面喷施硫酸铈可诱导增强硝酸钙胁迫下番茄幼苗叶片的抗氧化酶活性,有效降低膜脂过氧化程度,维持较高水平的光合性能,从而一定程度上缓解硝酸钙胁迫对番茄幼苗生长造成的伤害,增强其耐受盐胁迫的能力。

    Abstract:

    The present work studied the effects of 10 mg/L cerium sulfate on the antioxidant enzymes and photosynthesis of tomato seedlings ‘Hezuo 908’ leaves under 80 mmol/L calcium nitrate stress.The results showed that,comparing with the control,calcium nitrate stress significantly inhibited the growth of tomato seedlings by reducing the height,stem diameter,fresh weight,shoot and root dry weight.Furthermore,calcium nitrate stress also led to the decreased activities of SOD,POD and CAT as well as the decreased net photosynthetic rate,stomatal conductance,transpiration rate and intercellular CO2 concentration,whereas resulted in elevated MDA contents and membrane permeability.Comparing with the calcium nitrate stress treatments,however,after sprayed with cerium sulfate,some vegetative growth indicators,such as the plant height,stem diameter,fresh and dry weight were promoted in different degrees.In addition,the SOD,POD and CAT activities were also significantly up-regulated while MDA content and membrane permeability of leaves were greatly reduced.Meanwhile,the photosynthetic rate,transpiration rate and stomatal conductance were notably improved.Therefore,foliar spray of cerium sulfate helps to alleviate the damage caused by calcium nitrate stress through promoting the antioxidase activities,reducing the membrane lipid peroxidation and maintaining relative higher photosynthetic performance of tomato seedlings.

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黄守程,黄萍,陆晓民.硫酸铈对硝酸钙胁迫下番茄叶片抗氧化酶活性及光合性能的影响[J].西北植物学报,2015,35(9):1823-1828

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  • 在线发布日期: 2015-10-01
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