Abstract:Louisiana iris is a kind of high ornamental value of perennial plants,found to be tolerant and accumulation of heavy metal Pb.However,research on the molecular mechanism of Louisiana iris is limited.With the information of LiPCS1 iutermediate fragment form transcriptome of ‘Professor Neil’and using RACE technology,we cloned LiPCS1 for the gene bioinformatics prediction and analysis.The total length of the gene was 1 683 bp,predictive coding protein contained 501 aa.The protein contained three active essential amino acids sites,Cys,His and Asp,and base sequence of heavy metal ion sensor,C368C369RMTC373VRC376.The conservative analysis with other homologous PCS found that similar loci was 91.6%.The highest expression of LiPCS1 was found in inter petals of Louisiana iris,and significant differences with other organs.The same expression level was found in stamens,leaves and stems.The expression in outer petals,roots and pistils were lower,and the expression in pistil was the lowest.