A 2 100 bp sequence of OsPM1 promoter was obtained by searching in NCBI database.The promoter was cloned by PCR using DNA from rice as template.Cis-acting elements in this promoter were predicted by PLACE.The results showed that the promoter contains a large number of cis-acting elements associated with stress,such as ABRE,DRE,LTRE,HSE and binding motif of stress-associated transcription factor.The promoter of OsPM1 was cloned into expression vector pCambia1300-221-GUS to promote the expression of GUS gene and transgenic Arabidopsis was generated.GUS activity was detected in transgenic plants after drought,cold and high salinity treatments.The expression level of reporter gene was very low without treatment.After abiotic stress treatments,such as drought,low temperature and high salt,the expression of GUS increased significantly.The promoter can elicited the expression of genes downstream after abiotic stress treatments.