水稻淡黄绿叶色突变体的光氧化特性研究
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湖南省自然科学基金资助重点项目(12JJ2018);湖南省重点学科建设项目(湘教发[2011]42号)


Photooxidation Characteristics of the Yellow-green Leaf Mutant in Rice
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    摘要:

    为了解水稻淡黄绿叶色突变体‘标810S’的光保护机制,在自然强光条件下对突变体‘标810S’及其野生型‘810S’连体剑叶经5 mmol/L甲基紫精(MV)处理后的光氧化特性进行了比较研究。结果表明,在MV处理条件下,突变体‘标810S’剑叶净光合速率(Pn)、PSⅡ最大光化学量子产量(Fv/Fm)、PSⅡ实际光化学量子产量(ФPSⅡ)和光化学淬灭(qP)下降幅度明显低于野生型‘810S’,而非光化学淬灭(qn)上升幅度及PSⅠ和PSⅡ活性则明显高于‘810S’;同时,‘标810S’剑叶抗坏血酸(AsA)、还原型谷光甘肽(GSH)含量以及超氧物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)等抗氧化酶活性均明显高于‘810S’,而超氧阴离子(O2)产生速率、膜脂过氧化物丙二醛(MDA)含量则明显低于‘810S’。研究认为,在光氧化处理下淡黄绿叶色突变体‘标810S’是通过增加热耗散、提高抗氧化物质含量和保护酶活性、减少超氧阴离子和丙二醛的积累来维持较高的光系统活性和光合能力,从而表现出耐光抑制(光氧化)的生理特性。

    Abstract:

    ‘Biao 810S’ is a yellow-green mutant of the thermosensitive genic male sterile (TGMS) rice ‘810S’.To elucidate the mechanism of photoprotection in ‘Biao 810S’,we compared the photooxidation characteristics in leaves between ‘Biao 810S’ and ‘810S’ under natural light conditions with treatment of 5 mmol/L methyl viologen(MV).The results showed that the decreasing amounts of the net photosynthetic rate(Pn),maximal photochemical efficiency of PSⅡ in the dark(Fv/Fm),actual photochemical efficiency of PSⅡ in the light(ФPSⅡ),photochemical quenching(qP) in leaves of ‘Biao 810S’ were significantly lower than those of ‘810S’,but the increasing amount of non-photochemical quenching(qn),activities of PSⅠ and PSⅡ in leaves of ‘Biao 810S’ were significantly higher than those of ‘810S’.The contents of ascorbate(AsA) and glutathione(GSH),the activities of superoxide dismutase(SOD),catalase(CAT),peroxidase(POD),ascorbate peroxidase(APX) and glutathione reductase(GR) in leaves of ‘Biao 810S’ were significantly higher than those of ‘810S’,which led to a less endogenous superoxide anion generation rate(O2) and malondialdehyde (MDA) accumulation in leaves of ‘Biao 810S’ relative to‘810S’.Under the photo-oxidation treatment,‘Biao 810S’ can maintain higher light system activity and photosynthetic capacity by increasing thermal dissipation,antioxidant content,protect enzyme activities and decreasing accumulations of O2 and MDA,which showed a stronger tolerance to photoinhibition (photooxidation).

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肖辉海,郝小花,杨友伟,等.水稻淡黄绿叶色突变体的光氧化特性研究[J].西北植物学报,2015,35(11):2243-2250

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  • 在线发布日期: 2015-12-07
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