Abstract:Based on our previous study,CkLEA4,a Caragana korshinskii LEA gene induced by dehydration,salt and ABA treatments,was transferred into Arabidopsis. The transgene expression of eight T3 transgenic plants was detected by quantitative realtime PCR and three independent overexpression lines with different transgene expression level were selected for further analysis.For the germination assays,CkLEA4 overexpression lines germinated more quickly than that of wildtype on medium containing different concentrations of NaCl,mannitol or ABA.The germination rate of wildtype and CkLEA4 overexpression lines were all decreased with the increased concentrations of NaCl,mannitol or ABA,while the germination rate of wildtype decreased more dramatically than that of the overexpression lines.The cotyledon greening rates of CkLEA4 overexpression lines were also higher than those of wildtype under 200 mmol/L NaCl,400 mmol/L mannitol or low concentrations of ABA.Altogether,our results suggested that CkLEA4 improved Arabidopsis tolerance to salt,ABA and osmotic stress during seed germination stage.