F型小麦雄性不育系花粉败育和恢保关系研究
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Pollen Abortion of Ftype Male Sterile Line and Its Restorer and Maintainer Lines
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    摘要:

    F型小麦雄性不育系是一种新型“三系”小麦雄性不育系。为研究F型不育系的花粉败育特点并筛选其恢复源和保持源,采用I2KI染色法观察F、BNS、T、K和V型不育系扬花期的花粉败育类型,并以F型不育系为母本与98个优良小麦品种(系)进行杂交,检测F1代自交结实率。结果显示:(1) F型不育系的花粉败育率高达95.63%。其中,染败型花粉比例最高,达到67.66%;圆败率为19.32%;典败率最少,仅为8.73%。(2) 5种不育系中,F型与K型不育系的花粉育性特征最接近,其次是V型不育系。(3) 98个组合F1的自交结实率(国际法)在100%以上11个,0%~10%有18个。(4)‘周麦16’、 M510、‘西农815’、‘西农585’对 F型不育系的恢复力极强,是其优良恢复系;‘天麦989’、‘存麦4号’、CY 5475、M460、‘12漯1’和11GB02可通过回交培育成F型不育系的保持系。研究认为,F型不育系花粉败育彻底、稳定,在常规小麦品系中较易找到其保持源和强恢复力品系,是一种良好的新型不育系。

    Abstract:

    Ftype wheat male sterile line is a new “three lines” with karyoplasm interaction.For study the pollen characteristics of Ftype sterile line and screening its restorers and maintainers,the I2 KI staining method is used to observe pollens of F,BNS,T,K and Vtype wheat male sterile lines at blooming stage,and using 98 hybrid combinations that Ftype sterile line was female and 98 quality wheat lines were male to detect the self seedset ratios on F1 of combinations.Results showed that:(1) pollen sterility rate of Ftype sterile line reached 95.63% including the highest 67.66% stained aborted rate,19.32% spherical aborted rate and only 8.73% typical aborted.(2) The pollen sterility characteristics of Ftype was most similar to Ktype,the second was Vtype in 5 kinds of sterile lines.(3) In 98 hybridized combinations,11 selfseedset ratios (international) were more than 100% and 18 at 0%-10%.(4)‘Zhoumai16’,M510,‘Xinong 815’ and ‘Xinong 585’ were excellent restorer lines to Ftype sterile line.‘Tianmai 989’,‘Cunmai 4’,CY5475,M460,‘12 Luo1’ and 11GB02 were good maintainers for Ftype sterile line by backcross cultivation.Research suggests that Ftype male sterile line has thorough and stable pollen abortion and easily sought its maintainer resources and excellent restorers on fertility.

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张思妮,马小飞,张红霞,等. F型小麦雄性不育系花粉败育和恢保关系研究[J].西北植物学报,2016,36(4):667-673

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  • 在线发布日期: 2016-05-16
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