SRAP markers were used to study the genetic diversity and phylogenetic relationship for providind a theoretical basis for identification and crossbreeding of genetic resources of Lycium barbarum L.in Xinjiang of China. The results are: （1）a total of 310 loci were produced by 12 pairs of SRAP primers, and polymorphic loci were 264 which accounted for 84.61% in the total amplified fragments. The polymorphism information content (PIC) values of these markers varied from 0.76 to 0.93 with an average of 0.83. Mean values of observed number of alleles(Na),effective number of alleles (Ne),Nei’s gene diversity (H) and Shannon’s information index(I) were 1.846 1, 1.386 9, 0.228 0 and 0.352 0.（2）Range of genetic similarity (GS) was 0.590 3 to 0.903 2 among 30 wolfberry samples. Cluster analysis showed that samples could be divided into 2 groups and 5 subgroups accordingly at the GS of 0.70 and 0.76. The principal coordinate analysis and clustering results are basically consistent. Research shows the genetic diversity of germplasm resources of L. barbarum L. in Xinjiang was relatively abundant. The genetic difference between wild species and cultivars (lines) was relatively large,show a distant relationship, and the genetic difference among cultivars (lines) was relatively small, show a close relationship.