Abstract:To obtain bluish Lilium spp. in direct breeding, we screened the suitable varietyOT lily Robina for genetic transformation. Here, both embryonic callus induced from filament and regenerated bulb scales of OT lily Robina were used as the transformation material. Agrobacteriummediated transformation of Phalaenopsis F3′5′H was studied. The results showed:with the preculture 3 d, OD600 =0.8, infection time 10 min, cocultured for 3 d, 100 μmol/L acetosyringone conditions, the stable transformation rate of regenerated bulb scales could reach to the highest 12.78%; however, embryogenic callus preculture 2 d, OD600= 0.8, infection time 10 min, cocultured 3 d, with 100 μmol/L acetosyringone conditions, which the stable transformation rate was the highest 12.22%. In all the conditions, the best hygromycinresistant screening concentrations always was 20 mg/L. PCR and reverse transcription PCR assay showed 9 putative transgenic plants were obtained. Southern hybridization analysisfurther proved 6 plants that the transgenic lilium flowers carry blue gene F3′5′H. The results provided technical support and material basis for the continuing development of novel bluish Lilium flowers.