柳树β微管蛋白基因家族的克隆和序列分析
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国家自然科学基金青年项目(31400569)


Cloning and Sequence Analysis of βTubulin Gene Families in Willows
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    摘要:

    该研究克隆鉴定了旱柳和龙爪柳β微管蛋白基因,并对其进行了序列相似性、系统发育、染色体定位以及表达模式的分析。结果显示,2种柳树β微管蛋白基因家族各有20个成员,家族内部成员间核酸和氨基酸序列相似性分别在74.0%和86.6%以上,种间同源蛋白氨基酸序列相似性在85.8%以上,柳树与其它植物β微管蛋白间的氨基酸序列相似性在81.5%以上。系统发育分析显示,柳树β微管蛋白家族被分为4个亚组,结合杨树β微管蛋白基因染色体定位,推测柳树β微管蛋白基因家族经历了杨柳科全基因组重复事件和串联重复事件,而柳树TUB11TUB12可能来源于区段重复或者转座。基因表达模式分析发现,该家族成员的表达具有一定的组织特异性,并且部分重复基因对在所检测组织中表达差异较大。柳树β微管蛋白基因家族成员序列的高度相似性、成员数量的进化扩张、以及表达模式的多样性可能赋予了细胞分裂与生长更高的灵活性,这对多年生木本植物的生长发育习性意义重大。

    Abstract:

    In this study, βtubulin genes of Salix matsudana Koidz. and S. matsudana var. tortusoa (Vilm.)Rehd. were cloned and identified. The sequence similarity, phylogeny, chromosomal localization and expression patterns were further analyzed. The result showed that each willow contained 20 βtubulin genes, and each gene shared more than 74.0% and 86.6% nucleotide and amino acid sequence similarity with one another in each willow species. There was over 85.8% amino acid sequence similarity of the βtubulin between the two willows. While the amino acid sequence similarity among Salix and other plants were larger than 81.5%. In the phylogenetic analysis, βtubulin proteins formed four classes, and it was presumed that Salix βtubulin gene families had undergone salicoid duplication and tandem duplication events combined with the study of chromosomal location of poplar βtubulin. However, TUB11 and TUB12 were possibly derived from segmental duplication or replicative transposition. The expression pattern analysis showed a tissue specificity of βtubulin genes in willows, and a section of duplicatedgene pairs showed different expression patterns in tested tissues. High degree of sequence similarity, evolutionary expansion and of members and expression pattern diversity of Salix βtubulin gene families might confer flexibility in cell division and growth which is of important significance to the development and growth habit of perennial woody plants.

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睢金凯,饶国栋,张建国.柳树β微管蛋白基因家族的克隆和序列分析[J].西北植物学报,2016,36(5):902-909

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  • 在线发布日期: 2016-06-16
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