Abstract:Late embryogenesis abundant protein(LEA) are a suite of important family proteins found in organisms, which are generally involved in osmoregulation and are expressed abundantly when subjected to environmental stresses. In this study, we isolated a novel LEA3 gene TaDRLea32 from wheat‘Zhengyin No.1’. The full length of TaDRLea32 is 668 bp, whose ORF is 570 bp, encoding 189 amino acids, and consists of 9 conserved 11mer repeating motifs; Protein prediction shows that the protein is highly hydrophilic and located in the cytoplasm, with three phosphorylation sites and has no transmembrane domain or signal peptide; Secondary structure analysis shows that the main structure is alpha helix; Homology comparison and regenerating phylogenetic tree were carried out by MEGA5.1. Expression analyses revealed that TaDRLea32 gene was induced by drought, lowtemperature, highsalt stress and also moreover, can be induced by ABA treatment. These results suggested that TaDRLea32 is an ABAdependent LEA3 gene, participating in the response process of wheat under the abiotic stresses in different mechanisms and can lay a foundation for elucidating LEA3 gene protection mechanism under abiotic stresses in wheat.